If I prepare the following set of tests, incubate for 15 mins, then add 2-3 drops of iodine solution to each test tube: Salt test (1ml starch solution + 0.5 ml enzyme + 1ml 1M NaCl, incubate at 37 oC Acid test (1ml starch solution + 0.5ml enzyme + 1ml 0.5HCl), at 37oC Alkaline test (1ml starch solution + 0.5ml enzyme + 1ml 0.5M NaOH), at 37oC Temp test (1ml starch solution + 0.5ml enzyme + 1ml H2O), place in water-bath (75oC)

What is the effect of salt, pH and temperature to the salivary amylase? I don't know what exactly how salt effect. high temperature will denature the enzyme.

How about salt?

  • $\begingroup$ Sounds like a homework question to me. Homework questions are off-topic on Biology unless you have shown your attempt at an answer. For more information see our homework policy. "Homework" is interpreted to mean any academic or other assignment, test preparation, or task given in relation to a class, educational setting, or self-learning. $\endgroup$ Mar 31 '19 at 15:45
  • $\begingroup$ At extreme pH (too acidic or too alkaline), the salivary amylase will be denatured. At salt, the addition of salt will affect osmolarity and hinder the reaction. $\endgroup$
    – qweasd
    Apr 1 '19 at 6:06

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