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Taken from Ian Freshney's Culture of Animal Cells:

The enzymes used most frequently for tissue disaggregation are crude preparations of trypsin, collagenase, elastase, pronase, Dispase, DNase, and hyaluronidase...

What does crude mean in this context?

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In this context crude means naturally occurring or unrefined.

Purified trypsin alone, for example, is usually ineffective for tissue dissociation since it shows little selectivity for extracellular proteins.

Source: Worthington Tissue Dissociation Guide

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