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Does the yolk sac in general form always at the vegetal pole?
If so, is this the case for Danio rerio (the zebrafish)?

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Generally, yes, yolk is at the vegetal pole. There may be exceptions but I am not aware of them.

You can read about zebrafish (Danio rerio) embryology in great detail in this paper. Figure 3 from this paper (see image) shows the immediate post-fertilization zygote, in which the animal-vegetal axis is first set up by the migration of non-yolk to the animal pole.

figure3

UPDATE: very explicitly labeled yolk figures.

yolk poles yolk embryos

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  • $\begingroup$ Thank you! I read a paper but it was not clear to me where the yolk sac was eaxctly. $\endgroup$
    – user51074
    Apr 11, 2019 at 18:18
  • $\begingroup$ See new figures from paper in update. $\endgroup$ Apr 12, 2019 at 20:23

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