I stained a free-floating ~300uM brian slice using 4 different 1º antibodies (rabbit, chick, mouse, rat).
For the mouse primary, I mistakenly used an Alexa-350 secondary, when I should have used 594.
The microscope we have in lab cannot detect the 350 fluorophore, so I'm wondering if I can re-stain the slice with the anti-mouse 594 secondary.
Thanks for your advice !
[cross-posted in reddit/labrats]