Currently I am trying to read and understand this paper on intein splicing. https://sci-hub.tw/https://www.ncbi.nlm.nih.gov/pubmed/22001202

However, I'm a little confused with Figure 4.

enter image description here

Why do the authors have Fig. 4a and 4b, but it is not talked/referenced about anywhere in the paper?

I found:

"temperature does not visibly affect the splicing of highly efficient inteins"

Is that a reference to 4a? Furthermore, why is temperature important for some inteins but not for others? Why is this significant?

  • 2
    $\begingroup$ I'm voting to close this question as off-topic because for the most part it is not about biology as such, but about text references to figures in one particular paper which readers are expect to go away to read. SE is for self-contained questions *about biology which are likely to be of some general interest. As for the effect of temperature on inteins. If this is a prime concern it should be posted as a separate question. $\endgroup$ – David Apr 17 at 15:15
  • $\begingroup$ They didn't want to show all the inteins in a single figure. As you can notice in the figure legends, the highly efficient inteins are shown in panel (a) while the inefficient are shown in panel (b). I agree with @David You should have read the paper more carefully. As he says, if your question is about the temperature dependence, you should edit your question to focus on that part. However, that still means that you should do your part of reading the paper carefully before asking. $\endgroup$ – WYSIWYG Apr 25 at 8:07

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.