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I want to save my bacillus strains but I don't have access to a -80 degree freezer. What are possible alternatives?

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Use agar stubs: pour some Nutrient agar (or any suitable agar medium) in a test tube (~10% of the tube volume) and punch your inoculum halfway into it using a pipette tip or inoculation loop. Store it in 4⁰C.

Glycerol stocks can be made and stored in -20⁰C also.

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  • $\begingroup$ How long can you store them in agar stubs? Also we use -70 to -80°C to store our glycerol stocks. $\endgroup$ – NeoDevlin Jun 14 '13 at 17:03
  • $\begingroup$ I had stored them for a couple of months but i think it can withstand one more month.. $\endgroup$ – WYSIWYG Jun 15 '13 at 5:12

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