I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form separate colonies on an agar dish.
I know that because when I take the last 50 µl from a 1 ml tube and put it in a new tube and mix the 50 µL with a larger volume (e.g. 100 µL), we get a higher CFU count.

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    $\begingroup$ Just to be sure: is it really 10-50 cells in your pellet? Or is this a typo and its rather 10-50 x 10^6 cells or similar? $\endgroup$ – Arsak Apr 23 at 13:15
  • $\begingroup$ Thanks for the quick answer! Yes, there are only 10 to 50 colony building units in the 50 µL pellet! $\endgroup$ – Sam Apr 23 at 13:38
  • $\begingroup$ What homogenisation method(s) did you already try? $\endgroup$ – Arsak Apr 24 at 8:59

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