I developed a PCR cycle to amplify a 5.5 kb genomic DNA region in HEK cell line. When I tried to use the same protocol in A375 cell line I had no results but only smear. Can you help me? Thanks in advance

  • $\begingroup$ Are you running a housekeeping gene? how does that look like? $\endgroup$
    – Rover Eye
    Apr 24 '19 at 16:45
  • $\begingroup$ No, it's an intron of a gene upregulated in this cell line $\endgroup$
    – Annalaura
    Apr 24 '19 at 16:47
  • $\begingroup$ And in this intron there is an enhancer region $\endgroup$
    – Annalaura
    Apr 24 '19 at 16:49
  • $\begingroup$ Do you have a positive control run with your sample? $\endgroup$
    – Chris
    Apr 24 '19 at 16:56
  • $\begingroup$ I use HEK gDNA as positive control $\endgroup$
    – Annalaura
    Apr 24 '19 at 16:58

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