I am curious about the advantages of using single primer in PCR.

I found this strategy in single cell sequencing method Smart-seq 2.

Smart-seq2 library preparation steps

Will it raise the yield of PCR? If so, Why?

Does anybody have any idea of it? Thanks a lot.

  • $\begingroup$ Your question is unclear to me. What do you mean by single primer? $\endgroup$ – WYSIWYG Apr 25 at 12:25

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