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I am growing cell cultures at the moment, and I sometimes find 1-2 of these elongated, black structures in one of my 8 wells. Should I be concerned?

I have been growing these for about 5 days at this point, replacing the media every the other day. I figure that the life-cycle of mold is pretty fast, and that if it were truly mold that it would overpopulate the well pretty quickly. Perhaps it's just debris?

Potential Mold Second potential mold

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  • $\begingroup$ They could be pieces of tissue paper. Do these "things" grow after a while? Cell debris is generally smaller than the cells. Is there a picture with the cells in focus? $\endgroup$ – WYSIWYG May 8 at 13:04
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Most of the time you can find shards of plastic, defects in the plate and other non-cellular debris in brightfield that look suspicious. Even some cellular debris can look suspicious, like when your viability is really poor. That doesn't look like anything to me. You'd know if you had contamination because it would be in more than 1 field, you'd have more than 1-2 of them, and your culture would either change in color, turbidity or smell. If it's a plate defect, you probably wont see it move if you re-suspend the well, either.

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