Can monoclonal antibodies work only if the target of it is located in the cell's surface? or it can be used to targeting a protein located inside the cell?

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    $\begingroup$ You have been in this site for some time so probably you are aware of our site policy of expecting research effort from the user. Your question is very basic. Did you try to find out anything at all? $\endgroup$ – WYSIWYG May 8 '19 at 13:57
  • $\begingroup$ Your comment is probably related to your other question. $\endgroup$ – WYSIWYG May 8 '19 at 14:17

Monoclonal antibodies (or antibodies in general) are large proteins (around 150 kiloDaltons according to Wikipedia), so cannot cross the plasma membrane. So monoclonal antibodies target extracellular molecules or cell surface molecules. However, there are techniques to target proteins inside a cell, like lysing the cell, or using gene modifications, or otherwise a technique covered in this paper: https://www.tandfonline.com/doi/abs/10.1517/14712598.5.2.237

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