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SDS-PAGE and western blotting are both used to separate and isolate proteins. My question is, which technique should be used when? Does one of them has advantages over the other one?

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  • $\begingroup$ Blotting sits on top of SDS-PAGE, so it really depends on the question of your research which one you do... $\endgroup$
    – Chris
    Commented May 18, 2019 at 19:04
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    $\begingroup$ Please do some research before asking questions. Your premise is incorrect. $\endgroup$
    – David
    Commented May 18, 2019 at 19:06

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