Cloned a single herpesviral gene into pCDH-EF!-GFP and see phenotypic effect on cells of interest and viral gene transcripts BUT unable to detect ANY protein using HRP boosted western blot. Even with a 6-HIS or myc tag on the putative protein. Given that, is it feasible to proceed to add Bio-ID-2 to the viral protein and expect that 1. we still may see biotynylation, and 2. if we do it is sufficient proof that the viral gene is truly present albeit at low levels.

  • $\begingroup$ BioID is used to identify protein-protein interactions; I’m not sure why it would be of particular use in your case. $\endgroup$ – canadianer May 23 at 11:05

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