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I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (benthic mud) and cathode (salt water) have to be full of the agar-salt solution, or can it be partly full?

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Main function of the salt bridge is to conduct electrons. It should be in such a way that the agar within the PVC pipe from both end should be in contact with anode and cathode. so I would say keep it completely filled for better conduction.

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