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According to this link https://www.cell.com/cell-reports/pdfExtended/S2211-1247(14)00121-1

CD44 protein lose adherence to cell surface by energical agitation Apart from CD44, which proteins can lose adherence to cell surface by energical agitation?

that the speed and length of agitation during the collagenase digestion has a dramatic effect both on organoid yield and CD44 antigen presen- tation in the CD10+ cell fraction

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