I am using this buffer to preserve blood cells for long term storage.

The buffer contains the following: 100 mM Tris, 100 mM EDTA, 10 mM NaCl, and 0.5% SDS.

From the literature it seems that EDTA should not affect methylation patterns. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813389/

Does anyone know what to expect for SDS, Tris, or NaCl?

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    $\begingroup$ If this buffer is used the preserve cells then intracellular components should not be affected at all. As you can guess, Tris and NaCl are used for maintaining pH and tonicity respectively. I guess that 0.5% SDS is for preventing cells from clumping. $\endgroup$ – WYSIWYG Jun 23 at 13:51

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