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In order to get the yield of IgY through a set of purification steps, what method can I use?

Thanks in advice!

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  • $\begingroup$ Do you know the antigen of the Ig and can you make it and use it for assays? $\endgroup$ Commented Jun 29, 2019 at 23:02
  • $\begingroup$ Someone asked a similar question recently. $\endgroup$
    – WYSIWYG
    Commented Jul 1, 2019 at 9:47

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You can use western blotting with a anti IgY secondary antibody conjungated to a HRP (or other reporter), this method will primarily help with troubleshooting your purification and locating IgY in the different fractions and flowthroughs.

If you want to quantify, consider using an ELISA kit. Here is one such kit (https://www.antibodies-online.com/kit/1563240/IgY+ELISA+Kit/)

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