What kind of antigen is used to provoke/induce an immune response if you are trying to make therapeutic humanized antibodies for cancer and alzheimer's disease?

For example, if you wanted to make an antibody for tau targeting alzheimer's disease, how is that achieved from hybridoma technology? How do you induce the tau antigenic response?

  • $\begingroup$ This seems to have two questions in it, and it would be easier to answer if they were split apart, or if you clarified which one you're actually interested in. (1) How do you produce monoclonal antibodies to a given antigen? (2) How do you humanize monoclonal antibodies once they're produced? $\endgroup$ – iayork Jul 2 at 18:08

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