Can I simulate different patients by using different cell lines of the same cell type and from the same tissue? Can I also study apoptosis in an immortalised cell line?
I have a gene X (transcription factor) which from wet lab experiments induces apoptosis in cell type A (endothelial cells) and proliferation in cell type B (smooth muscle cells).
I want to address this differential behaviour using ChIP-seq of antibodies against gene X.
I do not have enough patient samples and I wish to purchase immortalised cell lines to perform ChIP-seq with 4 biological replicates per condition: untreated control and over expression of X.
I plan each biological replicate to be a different cell line of the same cell type and preferably the same tissue. Is it possible to have such?
I will also be very grateful if anyone provides me a link or good article containing the best practices in planning for a ChIP-seq experiment.
Thanks in advance.