We're working on invertebrate hemolymph (blood) and we have found with flow cytometry (staining with DRAQ5) a cell-like population without nucleus but it has RNA production.
Does anyone any experience with something similar? May anyone recommend any publication with something like that?
1) We don't know the real population size because we have identified it by flow cytometry, future experiments we wish to use TEM to characterize it better.
2) We know that this "population" produces RNA because we have extracted total RNA using low input RNA kit and run the result in Bioanalyzer.
3) We "know"/suspected this population doesn't have cell-nucleus because DRAQ5 is a DNA staining and this population is DRAQ5-. We wish study that by confocal microscopy ASAP.