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I am an intern in biology institute. I have a 17.3kbp plasmid need to transform to Ecoli. But I have tried many time but have no or very few colony on LB plate.

I know large plasmid have less frequency in transformation, How can I improve chance of successful transformation?

Thanks everyone.

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  • $\begingroup$ Welcome to Biology.SE! It is expected that you will demonstrate that you have done research on your own before asking questions here — for example you could start with this: researchgate.net/post/… …. –––––––– Thank you for taking the tour — for details on how to ask good questions on this site please see: biology.stackexchange.com/help/how-to-ask. Thanks! 😊 $\endgroup$ – tyersome Jul 26 at 4:30
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    $\begingroup$ Other than what tyersome has suggested, I want to add that questions that involve troubleshooting of lab experiments should include the detailed protocol used (or provide a link to a protocols article if it is exactly followed). $\endgroup$ – WYSIWYG Jul 26 at 8:53

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