Many protocols in my lab use sorbitol in buffers. For instance, in co-immunoprecipitation, we include it at a final concentration of 200 mM in our lysis buffer. I'm not entirely sure why though. I believe it might be as a crowding agent. But even if this is the reason, I'm not entirely sure what this means. Could someone explain?

  • $\begingroup$ @user1136 Please do not use comments for answers. $\endgroup$ – Bryan Krause Aug 23 '19 at 19:46

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