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Are there any marker enzymes present for ribosomes, nucleus and cell membrane. For mitochondria there are many, for lysosome it's cathepsin. I read about the marker enzymes of most of the other cellular components, but no information is there on these three. If no marker enzymes are present then how are ribosomes, nucleus and cell membrane are identified.

I am specifically looking for marker enzymes which can help identify the pellet component after subcellular fractionation.

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  • $\begingroup$ Why are specifically interested in marker enzymes? And could it be any enzymtically active proteins or are you looking for which activity can easily be measured? Ribosomes are catalytically active (ribo-)enzymes themselves and aren't actual compartments of the cell like all of the other things. The plasma membrane and nucleus are much more heterogeneous than mitochondria or lysosomes so they are usually identified by other markers than enzymes. $\endgroup$ – Nicolai Sep 2 '19 at 15:53
  • $\begingroup$ You need to describe the experimental procedure in which you need to check for these components. You can see nuclei and membranes under a microscope, and ribosomes are characterized by their components (look them up to see what these are), but I imagine that this may not be what you want. I suspect there is some incorrect premise underlying your question, but without the detail we cannot point it out. $\endgroup$ – David Sep 2 '19 at 16:43
  • $\begingroup$ If you are dealing with subcellular fractions, or are doing a subcellular fractionation by the method of de Duve, the ribosomes will be associated with the microsomal fraction. A good marker for microsomes is glucose-6-phosphate dehydrogenase as it is associated with the Endo Reticulum But I think you need to supply more expt detail. What fractionation method are you using, and to what end? (locate an enz/protein?) $\endgroup$ – user1136 Sep 2 '19 at 20:05

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