I am reviewing several MHC-peptide binding affinity predictors trained on IEDB data. Quantitative records for MHC class I allotypes come from a lot of different assays and, by extension, have different measurement types. Here are the most common measurement type descriptions (I haven't altered them in any way):

  1. dissociation constant KD (~IC50)
  2. dissociation constant KD (~EC50)
  3. half maximal inhibitory concentration (IC50)
  4. dissociation constant KD
  5. half maximal effective concentration (EC50)

All of the predictors I've reviewed so far consider these measurement types interchangeable, which I find extremely odd. While IC50 and EC50 can indeed be similar under certain experimental circumstances, I find it hard to believe that any one of these can be approximately equal to Kd (this site already has a post on this matter). I've contacted several authors and database curators, and all of them have assured me that these values can indeed be considered more-or-less interchangeable, but I can't find any references to support these claims.

  • $\begingroup$ Not an answer (I can’t comment yet), but what is the difference between 1, 2, and 4? $\endgroup$ – Markus Heller Sep 14 '19 at 3:24
  • $\begingroup$ @MarkusHeller as far as I understand, 4 means either 1 or 2. $\endgroup$ – Eli Korvigo Sep 15 '19 at 11:16

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.