0
$\begingroup$

I want to map a single N1-methyladenosine (m1A) modification by primer extension. I have silenced a gene which is responsible for guiding modification of m1A then I confirmed the silencing.

My next task is to check the level of m1A modification. For that many methods available but i wanted to do by primer extension with Use of dimethylsulfate to probe RNA. My question is if I follow dimethylsulfate method is it possible to detect m1A modification?

I am using total RNA. but I don't know how to prepare my RNA for this reaction.

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.