I want to map a single N1-methyladenosine (m1A) modification by primer extension. I have silenced a gene which is responsible for guiding modification of m1A then I confirmed the silencing.
My next task is to check the level of m1A modification. For that many methods available but i wanted to do by primer extension with Use of dimethylsulfate to probe RNA. My question is if I follow dimethylsulfate method is it possible to detect m1A modification?
I am using total RNA. but I don't know how to prepare my RNA for this reaction.
