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Which DNA polymerase (commercially available) can initiate polymerization at nicks, without a standard primer?

My goal is to perform rolling circle amplification starting at a nick.

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It sounds like you are looking for a DNA polymerase that can perform nick translation. NEB maintains a chart listing the properties of their polymerases, including nick translation and extension from nick. Without additional details, it looks like Taq would work for your purpose.

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  • $\begingroup$ Thanks for the answer. I was looking for something with strand displacement activity rather than 5’ removal. My goal is to perform rolling circle amplification starting at a nicking site. $\endgroup$ – alec_djinn Nov 26 '19 at 22:03

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