-1
$\begingroup$

I have an exercise problem . I have to calculate the number of cycles of a DNA sequence that is 420 bp . we use a buffer that contains 1.5ng dNTPs and the molecuar weight is 487.0 gr/mol and . now i know that im suppsed to solve it by the equation : n=log_2⁡〖(Xn/Xo)〗 . but I dont how im supposed to calculate the initial amount and the final amount

$\endgroup$
  • $\begingroup$ Based on the amount of dNTPs & the length of the DNA you can calculate how much dNTPs are used up in a given cycle (though technically you'd also need the length of the primers). However without a starting amount of DNA I don't see how it would be possible to calculate an actual number of final cycles. $\endgroup$ – Nicolai Dec 2 at 12:56
  • $\begingroup$ Welcome to Biology.SE! We generally encourage you to do some research on your own and then, informed by what you have learned, ask any questions you still have (ideally with references to reliable sources). For homework questions you are required to show your attempt to answer the question and to use the "homework" tag. ——— Please take the tour and then go through the help pages starting with How to Ask questions effectively on this site and edit your question accordingly. Thanks! 😊 $\endgroup$ – tyersome Dec 2 at 21:17