I have an exercise problem . I have to calculate the number of cycles of a DNA sequence that is 420 bp . we use a buffer that contains 1.5ng dNTPs and the molecuar weight is 487.0 gr/mol and . now i know that im suppsed to solve it by the equation : n=log_2⁡〖(Xn/Xo)〗 . but I dont how im supposed to calculate the initial amount and the final amount

  • $\begingroup$ Based on the amount of dNTPs & the length of the DNA you can calculate how much dNTPs are used up in a given cycle (though technically you'd also need the length of the primers). However without a starting amount of DNA I don't see how it would be possible to calculate an actual number of final cycles. $\endgroup$ – Nicolai Dec 2 at 12:56
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