Is it possible to ligate a small DNA molecule to mRNA? I was reading a protocol from Current Protocols in Molecular Biology and one of the procedures involve ligating a DNA molecule to mRNA using a DNA splint that overlaps between the 5' of the DNA molecule and the 3' of the mRNA.

Would a reaction like the following work?

5'-phosphorylated DNA linker mRNA library DNA splint T4 DNA ligase + buffer

  • $\begingroup$ T4 ssRNA ligase will work for this.. Check this page on NEB site. $\endgroup$
    Jul 1, 2013 at 1:47


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