CO2 is important for maintaining constant pH in cell culture. Unfortunately, I do not have access to such incubator and buying a new one is expensive. I found a cheap incubator online, but it doesn't support CO2. I'd like to ask if I can cultivate cheek epithelial cells in such incubator and if it won't negatively affect my cell culture. Another option is building a small CO2 generator inside the incubator. I made some calculations - I know how much CO2 I need to generate, how many grams and mL of solutions I need, moreover the build-up pressure is so small, it should not negatively affect the incubator.

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    $\begingroup$ Are you familiar with CO2-independent growth media? (I don't know anything about culture of that cell population specifically, though) $\endgroup$
    – Bryan Krause
    Feb 27, 2020 at 18:06
  • $\begingroup$ Thank you very much. I am not familiar with CO2-independent growth media, but I will take a look. I planned to buy a cheap DMEM from eBay. I hope, there are some inexpensive media you mentioned, or at least a method to convert DMEM to such medium. $\endgroup$ Feb 27, 2020 at 19:34

1 Answer 1


L-15 media does not require a CO2 atmosphere and according to Sigma-Aldrich this media is suitable for primary explants of adult human tissues.

  • $\begingroup$ Please take the tour and then consult the help pages for additional advice on How to Answer effectively on this site and edit your post accordingly. ——— Answers are much more likely to receive a favorable response if you include some details and supporting references (primary literature is best, but at a minimum a link to a description of this media). Without that support, your answer is indistinguishable from opinion. This is a good example of how to format references. Thank you! 😊 $\endgroup$
    – tyersome
    Feb 27, 2020 at 23:38

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