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I have 20 viral DNA samples collected for 6 time points. The DNA content varies over time My DNA extraction protocol step suggests that in the last step of the protocol during elution I had 100uL of the solution. I accidentally added 200 uL of the solution for one time point during elution.

For all time points, I ran qPCR with the same amount of DNA (3uL) with SYBR Master Mix (22 uL). How do I correct the qPCR reading for the time point where I added 200uL of solution instead of 100uL?

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