my title is not very specific. So i will proceed to clarify it. I am trying to make sure that the only blood cells in a sample are Erytocytes, since i want to evaluate their metabolism, I am aware that they don't have Mithocondria and thus catabolize Glucose into Lactate, (I belive I am not making false statements, however if any corrections pop up please give me a heads up.) Thus a way I thought of getting rid of leucocytes was through centrifugation, erythrocytes would be at the bottom of the flask and if We got rid of Supernadant (plama and fine layer of leucocytes), we would have a much purer sample of erythrocytes.
My question is: Do they stil carry out their metabolism, so i can a posteriori evaluate it, even after centrifugation and gettting rid of the plasma. Or evaluating the lactate production afterwards would be impossible? I know there are more easier and methodic ways of getting rid of leucocytes(irradiation that destroys all nucleated cells and leucoreduction by filtracion, for example), but this is somewhat hypothetical, and i need to know the final answer in these circumstances.
Thanks in advance :)