Random Amplification of Polymorphic DNA (RAPD) is a widespread method to identify species or strains that are otherwise difficult to tell apart (microorganisms, tiny insects and the like). It includes running PCR reaction with the mix of randomly produced primers. Due differences in DNA sequence, gel bands of the PCR product have different pattern for different subjects we wish to identify.
I am interested in, how is the typical laboratory picking they primer set. I think it could be the following cases:
- Most of laboratories use commercial primer sets like made by various companies.
- There are non commercial primer sets produced and shared by academic community. Some of them are standard and widespread.
- It is common to start the larger project from producing the own specific primer set.