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I would like to ask how i can search in blast against UNIPROT (TREMBL PLUS SWISSPROT) for a selected organism. In the BLAST site there is no option about UNIPROT(only Swissprot), and in the uniprot site i cannot limit the results only in one organism(E.COLI). THANKS A LOT!!!

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  • $\begingroup$ Welcome to Biology.SE! Please take the tour and then go through the help pages starting with How to Ask questions effectively on this site. In particular, this question seems like it might be a better fit for Bioinformatics. Thanks! 😊 $\endgroup$
    – tyersome
    Apr 26, 2020 at 0:07
  • $\begingroup$ thank you and i am sorry!! $\endgroup$
    – marilu
    Apr 26, 2020 at 7:04

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You can blast for E.coli proteins only using Uniprot. Firstly, you restrict the search to the "target database" UniprotKB...Bacteria. After performing the blast search, you can further restrict your search results to include only hits for one "Popular organism", e.g. "Escherichia".

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  • $\begingroup$ thank you so much!! $\endgroup$
    – marilu
    Apr 26, 2020 at 15:51
  • $\begingroup$ @marilu Can you please mark my answer as correct? $\endgroup$
    – DaRealHonk
    Apr 30, 2020 at 9:16
  • $\begingroup$ is it ok?i think i did it $\endgroup$
    – marilu
    Apr 30, 2020 at 9:38
  • $\begingroup$ @marilu Yep, thank you! $\endgroup$
    – DaRealHonk
    May 2, 2020 at 6:05

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