I've seen Taq polymerase being marketed as either "native" or "recombinant". I understand that the recombinant version is produced by specially modified Escherichia coli strains that have the gene for polymerase production spliced in them, but I am wondering about the "native" variant. I imagine that Thermus aquaticus is rather difficult to culture in a medium; is it thus true that the archaeans have to be extracted from the springs they came from for producing "native" polymerase?
For recombinant Taq polymerase, industrial-scale production produces liters of highly concentrated enzyme in a single run. It comes in such small, dilute quantities when sold that only a few preps a year would be necessary to satisfy research lab demand.
I don't know the Thermus aquaticus protocol, but considering the achievements in yield for E. coli, and the minimal price difference between the two enzymes, I'd imagine that yields are similar there. That would say to me that nobody's farming hot springs for T. aquaticus.
protected by Chris♦ Mar 18 '15 at 20:20
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