what are the plausible reasons as to why the HRPO-conjugated donkey anti-human immunoglobulin and the chromogen/substrate were added from the right to the left of the microtiter plate?

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    $\begingroup$ Unfortunately this question is impossible to understand. It sounds like a context-less homework question. Please re-write it entirely, otherwise it will be closed. See the help section on how to ask a question. $\endgroup$ – S Pr Jun 25 '20 at 12:44

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