If we denature dsDNA by heating it and then rapidly cooling it then what would happen? I read this question, where it was written that if we were given dsDNA sample which was completely denatured and then if we rapidly cool it. What would the result be? Would it anneal rapidly too or the annealing will be slow?
- If we see PCR the annealing time varies but is around 45 sec min with temperature range from 55-65 °C ( depending on Tm value). So my first doubt is that is it called rapid cooling?
- In case of preserving the RNA or DNA- cooling on ice pack preserves its structure and prevents any further changes in structure also.
- I tried to read papers and I read somewhere thatif we completely denature it then it anneals slowly. That takes place in two steps- first, it will try to find its completely match by collision process. Second, it will zipper up. That takes around 55 mins. So that states that it will be rapid annealing.