When transforming a K. pastoris strain, is there a way to predict how many copies of the gene will be integrated following the homologous recombination? More specifically is it something that a molecular biologist is able to control in a simple way, or, is it usually left to the chance (and determined afterward using gene sequencing)?
Alternatively : if I want to transform an electrocompetent wild type strain such as Pichia X33 using a plasmid vector, is the multiple integration an iterative process I control or is it random?)
Edit: Thank you all for the answers. Finally if I understand well the process is pretty random but you can control it to some extent. @gaspanic: I keep the idea of using fluorescent reporters and FACS for that ;)