I am attempting to use CRISPR to insert a tag into a gene. The way we have done this in the past is to use separate crRNA (with a sequence to interact with the tracr) and the universal Tracr sequence seperately. This is then injected with an isolated Cas-9 protein and repair template. My question is can one use a single oligo sgRNA (crRNA+entire Tracr) interchangeably, or do you need a modified Cas9 to accept the single oligo version gRNA.


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