I would like to better understand, if possible, what kind of RNA molecules exosomes transport in human cells.

The experiment behind this general question is our goal to do a combination of mRNA-Seq and miRNA-Seq. We would like to be able to prepare libraries for sequencing from the same samples to reduce possible bias as good as it gets. For that we would like to extract both RNA and miRNA from the exosomes, if possible in one run.

Is there a way to approximate the ratio of how much small RNA molecules compared to MRNA is transported by exosomes?

How much of the small molecules transported by the vesicles are truly just fragmented mRNA and not real small RNA (e.g. miRNA, snRNA etc.)?

If anyone is aware of a paper/research regarding this direction, I would appreciate the citing.

thanks Assa


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