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I don't have a lot of experience in the design of animal experiments.

Let's say factor X that is required in humans for the generation of long-lived plasma cells.

This is a completely theoretical questions, so I cannot provide you with information about factor X. I just want to know what I should theoretically.

I generate a mouse model and delete factor X, but 6 months old, non-immunized mice don't show a significant change in total serum immunoglobulins or plasma cell numbers in the spleen or bone marrow.

How should I proceed? My first thought is to do a BLAST search for a homologous gene to factor X in the mouse genome. Maybe a factor Y has the same function as factor X. Deleting only Y should have the same as the deletion of X. A second experiment which deletes X and Y should then have the desired effect.

Is there anything else that would make sense?

Also, I think 6 months is quite young for mice, yes? So maybe factor X becomes only relevant later in life? If that is true, then observing the mice longer should so a decrease in plasma cells.

I hope the question is clear. Please let me know, if I should change it.

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  • $\begingroup$ Welcome to Biology.SE! Please take the tour and then go through the help pages starting with How to Ask questions effectively on this site and edit your question accordingly. In particular, hypothetical questions lead to opinions rather than factual answers and thus are off-topic for this site. Also note that each question should be posted separately — this improves the chances that you will get answers for each question and makes the answers more accessible for future users. Thanks! 😊 $\endgroup$ – tyersome Oct 17 at 17:47

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