What does it mean by adding PAN before the AMPK. I am trying to do a western blot assay on AMPK and I am confused by PAN-AMPK, AMPK. does it mean pancreases? Is pan-ampk the total ampk?

  • $\begingroup$ Welcome to Biology.SE! Please take the tour and then go through the help pages starting with How to Ask questions effectively on this site and edit your question accordingly. In particular, you need to be clear — e.g. abbreviations must be defined. We also expect you to do some research on your own and then, informed by what you have learned, ask any questions you still have (ideally with references to reliable sources). See also this sites criteria for "homework", which can also apply to questions that aren't assigned as homework. Thanks! 😊 $\endgroup$ – tyersome Oct 21 at 23:33
  • $\begingroup$ Can you please give some more background of your research? $\endgroup$ – Chris Oct 22 at 5:54

Adenosine monophosphate activated protein kinase or AMPK is not a single protein, but is a trimeric enzyme composed of $\alpha$, $\beta$, and $\gamma$ subunits. There are 2 alpha genes, 2 beta genes, and 3 gamma genes, each coding for closely-related but not identical isoforms. In addition, like most protein kinases (and many other proteins), it is regulated by the phosphorylation and dephosphorylation of some of its residues, most notably Thr172, but at numerous other locations as well.

A "pan-AMPK" antibody will bind all of the AMPK isoforms independently of phosphorylation or other post-translational modification (PTM) state. There are also isoform-specific total AMPK antibodies that just bind the $\alpha2$ subunit, or all 3 $\gamma$ subunits, for example. Finally, there are phospho-specific antibodies to various residues that only bind when the protein is phosphorylated at that site.

Both the pan and isoform-specific antibodies are known as "total" antibodies, as they bind independently of PTMs and will show the total amounts of their target(s) on a Western blot, for example.

Source: I used to be a Product Scientist for a company that makes antibodies, PTM-specific and total, to AMPK and many other targets.

| improve this answer | |

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.