I am interested in extracting aminoacids from microalgae after having ruptured its membrane and carried out proteolysis. After some research, I came to the conclusion that ion exchange chromatography is the best for a medium scaled extraction of aminoacids. However, considering that the tool is quite prohibitive, I was wondering if it were possible to make a small scale DIY version of it, in order to, get a feeling of how it works and what parameters it needs. Even though, I am not sure this question can be asked here, I do wonder how I could make a DIY ion-exchange chromatography system. Even though, a straight-forward answer would be appreciated, I would really value just to hear small tips on it.
Other "side" doubts:
- How can I "stick" the ionic resin to the tube's wall?
- Do you know of any online sources that will help me give the system the correct pH parameters in order to achieve optimum net charge?
- Am I not approaching this correctly, is ion exchange chromatography not the solution?
I do apologize if this question isn't meant to be here. If that is the case, I would be grateful to have the question migrated elsewhere. I will only need to extract these 20 aminoacids (which are, basically, the 20 aminoacids in human metabolism).