During autoclave heat-up, the inside chamber's air is released in order to be displaced by steam. Because of that, many modern autoclaves are equipped with filters at the release outlet. The aerosolisation is understandable when working with potentially dangerous bacteria and viruses. However, are mammalian cells (used, for instance, in gene editing cell cultures) capable of aerosolisation as they are significantly larger in size compared to bacteria? If so, is the frequency of mammalian cells aerosolisation higher or lower than in bacteria?

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    $\begingroup$ One consideration for mammalian cells is that the media they are grown in tend to be pretty hospitable to bacteria. So if you're autoclaving cells that have sat in a biohazard bin for days (or maybe weeks), it's likely that you're autoclaving bacteria as well. $\endgroup$ – MikeyC Oct 26 '20 at 15:03

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