Im having trouble understanding Scatchard plots.

Y Axis = Bound/Free Ligand

X Axis = Bound Ligand

The graph has a negative slope.

Why when there is almost no Bound (Y axis = 0) do we get a high positive number for Bound ligand on the X Axis corresponding to Receptor concentration? if there is no bound how is there a positive bound number?

it looks like the less bound ligand on the Y axis, the more bound on the X axis!

enter image description here

  • $\begingroup$ can you pls provide a link to the paper? also the bound/free can be ratio of occupied sites and not ligand but i will have to see in the paper. $\endgroup$
    – Roni Saiba
    Nov 29 '20 at 2:33

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