Im having trouble understanding Scatchard plots.
Y Axis = Bound/Free Ligand
X Axis = Bound Ligand
The graph has a negative slope.
Why when there is almost no Bound (Y axis = 0) do we get a high positive number for Bound ligand on the X Axis corresponding to Receptor concentration? if there is no bound how is there a positive bound number?
it looks like the less bound ligand on the Y axis, the more bound on the X axis!