Context: I have seen promoters used to have gene therapy viruses target specific cells, so I am referring to that apparent function of theirs. As an example I have seen GFAP promoters used for targeting astrocytes (I have seen this in many more papers but this is the first one I found on google).
I am curious what the purpose of the EF1-alpha promoter in a lentil/retro/Adeno-Associated virus for gene expression is?
It would seem that it is often used for targeting embryonic stem cells, but then I had also seen else where that "The Lenti-X Expression System (EF1α Version) enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentiviral transduction." This made me think that perhaps since this promoter 'targets' stem cells which later turn into any type of cell, that this quote meant that the EF1-alpha promoter can be used to indiscriminately target cells? Is this true?
Please forgive any incorrect assumptions on my part, I am a novice in this field.