I need to understand the level of RNA over-expression that is normally reached with HEK293 T-REx Flp-In cells. This over-expression system is widely used in PAR-CLIP experiments, which is why I want to understand it. I asked some gene-expression experts, but so far nobody give an estimate. Here I would like to ask for a reputable source, while "gut-feeling" estimated would be helpful as well.
Additionally, to give an idea how I tried to approach this question, here I found a data-series that could finally reveal the answer:
Expression of FLAG/HA-LIN28A or FLAG/HA-LIN28B was induced by addition of 250 ng/ml doxycycline 15 to 20 h before crosslinking.
This contains 2 replicates of RNA-seq with and without induction of LIN28A or LIN28B. So the idea is to ascertain the expression level of LIN28A/B with or without induction, relative to the whole transcriptome. However, the file uses read identifiers like "213084_x_at" instead of transcript/gene names/IDs, and I don't know how to map them to genes automatically.
Similar to this idea, has anyone maybe read any other papers where HEK293 T-REx Flp-In overexpression is performed, that measure the RNA levels?