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I'm building an app which helps researchers plan out Western blots, record protocol tweaks and receive warnings in the app if:

  1. you're trying to multiplex antibodies of similar molecular weight on the same membrane

  2. you're using multiple antibodies on the same membrane for which different tweaks have been previously used leading to a "procedure conflict"... The app will suggest a membrane cut to allow the optimizations to be applied.

Are there other common tweaks that you make to your Western blot setup that you find yourself constantly needing to look up and/or forgetting?

I'm looking for a common use case to demonstrate the app's utility.

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