What is the mechanism by which calcium ions and heat treatment allow the bacterial membrane to become permeable, allowing the uptake of plasmids?


DNA has an overall negative charge. The phosphate heads of the lipid molecules that make up the cell membrane, along with the LPS molecules on the outer membrane, are also negatively charged. This means the plasmid is normally repelled by the cell membrane.

What Ca2+ ions do is shield the negative charges on each side, making the overall situation electrostatically neutral.

This site has a nice animatic for this process: http://www.dnai.org/b/index.html

  • $\begingroup$ Thanks owlofathena $\endgroup$ – k Suraj Mar 5 at 6:32
  • $\begingroup$ There are two things that need to be corrected in your answer. The first is that the effect of calcium is on the bacterial cell wall, not the cell membrane. The second is that "lipid" should be replaced with the specific membrane constituent of Gram-negative bacteria — lipopolysaccharide. There is a short Wikipedia entry for calcium chloride transformation. $\endgroup$ – David Mar 5 at 17:39
  • $\begingroup$ You're right that I should have included LPS along with phosphate groups as contributing to the negative charge on the cell surface. That was sloppy of me. Edited. Thanks! $\endgroup$ – owlofathena Mar 6 at 7:33

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