Questions tagged [antibiotic-resistance]

Tag for questions related to the ability of bacteria to adapt to antibiotic agents in there environment through genes present in their genome or acquired from the DNA of other organisms through the process of Transformation.

Filter by
Sorted by
Tagged with
3 votes
1 answer
69 views

Is bacterial resistance caused by underdosing?

The phenomenon of antimicrobial resistance caused by overuse of antibiotics was predicted as early as 1945 by Alexander Fleming who said "The time may come when penicillin can be bought by anyone ...
user avatar
2 votes
0 answers
30 views

How much Kanamycin is too much for selection in dh5α E. coli?

I'm using Kanamycin for selection after electroporation of my plasmid containing a kanamycin-resistance gene at a concentration of 50mg/L prepared in 1000x stock that is usually kept in frozen ...
user avatar
  • 327
1 vote
0 answers
68 views

Do bacteria select for "soap resistance"?

When people wash their hands with soap, this limits the spread of bacterial infections. That much, I am pretty convinced of. Duh. What I don't get is how this doesn't lead to evolutionary pressure on ...
user avatar
  • 119
0 votes
0 answers
38 views

Why is syphilis not resistant to penicilin?

How is it that we are dealing with so many antiobiotic resistant infections in iatrogenic settings, and yet this very old bacterial pathogen has still not acquired resistance to penicilin (the oldest ...
user avatar
  • 11
0 votes
0 answers
32 views

Why does activated charcoal prevent tetracycline activity in E. coli culture?

My lab just found that using activated charcoal in an E. coli culture prevents tetracycline function. What kind of reaction could be causing this effect? Thanks!
user avatar
0 votes
0 answers
17 views

Are the kind of mutations different in an antibiotic setting than they are in a non antibiotic setting?

Hy everyone! I'm looking for experiments that deal with the kind of mutations that arise in antibiotic environment, as opposed to non antibiotic environment. As far as I understand, AB (anti biotic) ...
user avatar
14 votes
1 answer
947 views

Does cell culturing contribute to dangerous antibiotic resistance to the same degree as livestock?

According to the CDC, antibiotic resistance is one of the biggest public health challenges of our time. The largest incubator for antibiotic resistance is the factory farming of animals where ...
user avatar
5 votes
2 answers
277 views

In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
user avatar
  • 141
1 vote
1 answer
63 views

Can I find out what resistance bacteria has based on strain? where do I look for that info?

I have a list of strains across various organisms for example: Escherichia coli - ATCC 25922, CGSC 6152, W3110RL. Pseudomonas aeruginosa - ATCC 27853, PAO1, APAE1111. I'm failing to find any ...
user avatar
2 votes
1 answer
102 views

Why would a mammalian vector plasmid require an antibacterial resistance gene?

I have been trying to understand the composition of plasmids used in recombinant DNA cloning, such as this: https://www.genecopoeia.com/wp-content/uploads/2020/02/pReceiver-M35-051818.pdf and I am ...
user avatar
2 votes
1 answer
110 views

If mutations are random, why do antibiotics 'cause' resistance?

Full disclosure: I am not a biologist and this is more of a general question about understanding antibiotic resistance of bacteria. We are always told that careless antibiotics use is the cause of ...
user avatar
44 votes
6 answers
12k views

How do scientists kill the bacteria they themselves made resistant?

I was reading this article on researching bacteria resistance to silver by removing some of their genes. Researchers then used "colony-scoring" software to measure the differences in growth and ...
user avatar
  • 501
0 votes
2 answers
149 views

Can you design a bacteriophage that attacks the part of the bacteria that makes them antibiotics resistant?

I read in a newspaper about a case where they had found a virus that could attack a specific form of antibiotics-resistant bacteria and managed to save a girl from a certain death by "infecting her" ...
user avatar
  • 532
0 votes
0 answers
41 views

Antibiotic resistant genes

Can someone help me find be it an article or journal that has the conventional PCR method for the detection of antibiotic resistant genes? Thanks in advance.
user avatar
3 votes
2 answers
355 views

If bacterial resistance randomly occur, then why limit broad-spectrum antibiotic use?

If there is importance to study some discipline, then one of the main matters is its applications, so besides the primary goal of knowing the truth of the matter regarding what that discipline is ...
user avatar
2 votes
1 answer
90 views

Can (or have) antiviral drugs created drug-resistant viruses?

Evolution/emergence of antibiotic-resistance in bacteria is a known effect of extensive use of pharmaceutical antibiotics. Pharmaceutical antivirals have come into extensive use in recent decades – e....
user avatar
  • 703
2 votes
1 answer
108 views

Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
user avatar
5 votes
1 answer
106 views

Are there limits to drug resistance?

It's well-known that indiscriminate use of a drug leads to resistance. My question is what the limits on resistance are. It seems obvious that there must be limits: for example I can hardly imagine a ...
user avatar
  • 469
1 vote
1 answer
17 views

How close are we to having phages take over the role of infection control from antibiotics? What developments need to occur?

The discovery of, and research in, the field of phages was overshadowed by the discovery and implementation of antibiotic treatments for infectious disease control. With the rising concern of ...
user avatar
4 votes
1 answer
78 views

Why is AMR not widespread?

we know that resistant strains (MDR, XDR, PDR) have arisen in single incidents around the globe but why are these bacteria not common and not spreading quickly as humans keep using antibiotics and ...
user avatar
1 vote
2 answers
79 views

Is it true that it is mostly impossible, for any kind of antibiotic, to succeed in killing off every bacteria in a very rich environment?

It is commonly believed that the resistance to antibiotics by micro-organisms is truly evolution at work, and that the recent surge in superbugs may very well be attributed to it. When we refer to a ...
user avatar
  • 31
3 votes
2 answers
67 views

What effect does taking antibiotics unnecessarily have on future bacterial infections?

I have been told by my biology teacher that you shouldn't take antibiotics unnecessarily because "the body gets used to them" and it reduces the effectiveness of antibiotics against future bacterial ...
user avatar
0 votes
1 answer
55 views

Is a wild-type strain more "fit" than its mutant strains?

Question's context In the context of a presentation on mathematical models for antibiotic-resistance bacteria research, someone mentioned that wild type strain (WT) are expected to be more fit than ...
user avatar
  • 13
4 votes
1 answer
179 views

Test of antibiotic A for sensitivity as surrogate of antibiotic B

I am reading the WHO Global Antimicrobial Resistance Surveillance System (GLASS) - Manual for Early Implementation. it mentioned some of the drug-bug combination that will be put under surveillance in ...
user avatar
  • 113
-1 votes
2 answers
109 views

Why antimicrobial resistance is based mainly on antibiotic use? [closed]

Why antimicrobial resistance is based mainly on antibiotics use and not so much on antifungal or antivirus drugs? Why, when someone speaks about antimicrobial resistance, refers mostly to antibiotitic ...
user avatar
  • 1
3 votes
0 answers
114 views

Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?

Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
user avatar
1 vote
3 answers
556 views

Could plasmids and conjugation mechanisms be used against antibiotic-resistant bacteria? [closed]

I'm surprised no one has mentioned something like this. Plasmids are often exchanged between bacteria, sometimes through conjugation. In particular, conjugation could be considered an "open-port&...
user avatar
1 vote
0 answers
23 views

How quickly do bacteria lose genes after selection has been removed? [duplicate]

If bacteria are subject to a biocide and then that biocide is removed from the population, how quickly will they lose that gene that encodes for resistance?
user avatar
2 votes
1 answer
144 views

Why would eating the meat of cows fed with antibiotics trigger an immune response to the antibiotics? [closed]

The document "Antibiotics and Antibiotic resistance" contains the following paragraph: The indiscriminate use of antibiotics in feed stuffs means that humans may receive unwanted doses of ...
user avatar
2 votes
1 answer
84 views

Have bacteria evolved antibiotic resistance in a laboratory setting?

Is it possible to breed a strain of bacteria in a laboratory setting so that it gains resistance to an antibiotic, replicating the kinds of resistance seen in wild, and has that been done?
user avatar
0 votes
2 answers
48 views

What placement of plasmid parts makes the most sense?

If you want to express the largest amount of a certain or a few proteins using a plasmid, does it make sense for the antibiotic resistance gene to come after genes for proteins you intend to ...
user avatar
  • 371
2 votes
1 answer
554 views

Will dead cells in culture media have any significant impact on Live cells during incubation?

I am generating stable cell lines and have something like 25+ culture flasks to take care of. Ideally I can let dead cells sit in the media with the live adherent without rushing to change media ...
user avatar
4 votes
2 answers
564 views

Resistance to Antiseptics

I know that bacteria can become resistant to antibiotics. Why do antiseptics like alcohols, PVP-I, still work on the strains of bacteria we have today? Shouldn't some bacteria have evolved to be ...
user avatar
-1 votes
1 answer
696 views

Drug and antibiotic resistance in organism [closed]

Out of gram positive or gram negative or both which one is having a higher resistance to drug or antibiotics? How the organism gains that gene for drug or antibiotics resistance.? Kindly explain it in ...
user avatar
  • 369
4 votes
1 answer
561 views

What does completing the course of antibiotics accomplish?

This article from the WHO states: If you stop treatment early, there is a risk the antibiotics won’t have killed all the bacteria that made you sick and that it will mutate and become resistant. This ...
user avatar
  • 1,863
2 votes
0 answers
62 views

After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
user avatar
  • 121
-2 votes
3 answers
2k views

Why do we take antibiotics if our immune system already produces them? [closed]

Why do we take antibiotics if our immune system already produces them? Is it because our body doesnt make enough or the specific complementary antibody to fit with the antigen?
user avatar
  • 21
3 votes
1 answer
245 views

Why can bacteria resist topical antibiotics, but not compounds like Benzoyl Peroxide?

I excerpt from p 2 of 4, but p 4 of 4 reviews the entire article by Debra Jaliman, MD on Apr 17 2014. Usually, topical antibiotics aren't recommended alone as an acne treatment, as they can increase ...
user avatar
11 votes
1 answer
2k views

Why don't vaccines cause bacterial resistance?

Since bacteria can evolve to overcome antibiotic use, why wouldn't they be able to evolve to overcome antibody or cell-mediated immunity? (One possible explanation: antibiotics have only one target ...
user avatar
  • 961
3 votes
1 answer
649 views

Does long term use of antibiotics in humans actually lead to a greater risk of infection?

I've read about the overuse of antibiotics leading to antibiotic resistant strains of bacteria, so generally does long term use of antibiotics breed these strains in the bodies of antibiotic users and ...
user avatar
1 vote
1 answer
569 views

Which immunosuppression caused by long-term usage of these antibiotics in untreated celiac disease?

These antibiotics include amoxicillin + acidum clavulanicum (beta-lactam, inhibit cell wall synthesis, broad specturm, 3rd gen aminopenicillin) / last part preventing the resistance ceftriaxone (3rd ...
user avatar