Questions tagged [antibiotic-resistance]
Tag for questions related to the ability of bacteria to adapt to antibiotic agents in there environment through genes present in their genome or acquired from the DNA of other organisms through the process of Transformation.
41
questions
0
votes
1
answer
23
views
Can disk diffuson be used, without diluting a broth culture to match a 0.5 McFarland turbidity standard?
In this video, there doesn't seem to be a process of diluting the broth. I'm not looking for extremely accurate test results, all I need is to see if a particular colony is susceptible to the ...
3
votes
1
answer
78
views
Is bacterial resistance caused by underdosing?
The phenomenon of antimicrobial resistance caused by overuse of antibiotics was predicted as early as 1945 by Alexander Fleming who said "The time may come when penicillin can be bought by anyone ...
- 195
2
votes
0
answers
119
views
How much Kanamycin is too much for selection in dh5α E. coli?
I'm using Kanamycin for selection after electroporation of my plasmid containing a kanamycin-resistance gene at a concentration of 50mg/L prepared in 1000x stock that is usually kept in frozen ...
- 327
1
vote
0
answers
110
views
Do bacteria select for "soap resistance"?
When people wash their hands with soap, this limits the spread of bacterial infections. That much, I am pretty convinced of. Duh.
What I don't get is how this doesn't lead to evolutionary pressure on ...
- 119
0
votes
1
answer
102
views
Why is syphilis not resistant to penicilin?
How is it that we are dealing with so many antiobiotic resistant infections in iatrogenic settings, and yet this very old bacterial pathogen has still not acquired resistance to penicilin (the oldest ...
- 11
0
votes
0
answers
18
views
Are the kind of mutations different in an antibiotic setting than they are in a non antibiotic setting?
Hy everyone!
I'm looking for experiments that deal with the kind of mutations that arise in antibiotic environment, as opposed to non antibiotic environment. As far as I understand, AB (anti biotic) ...
14
votes
1
answer
956
views
Does cell culturing contribute to dangerous antibiotic resistance to the same degree as livestock?
According to the CDC, antibiotic resistance is one of the biggest public health challenges of our time. The largest incubator for antibiotic resistance is the factory farming of animals where ...
- 193
5
votes
2
answers
612
views
In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?
The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why.
I ...
- 135
1
vote
1
answer
67
views
Can I find out what resistance bacteria has based on strain? where do I look for that info?
I have a list of strains across various organisms for example:
Escherichia coli - ATCC 25922, CGSC 6152, W3110RL.
Pseudomonas aeruginosa - ATCC 27853, PAO1, APAE1111.
I'm failing to find any ...
2
votes
1
answer
142
views
Why would a mammalian vector plasmid require an antibacterial resistance gene?
I have been trying to understand the composition of plasmids used in recombinant DNA cloning, such as this: https://www.genecopoeia.com/wp-content/uploads/2020/02/pReceiver-M35-051818.pdf
and I am ...
- 83
2
votes
1
answer
137
views
If mutations are random, why do antibiotics 'cause' resistance?
Full disclosure: I am not a biologist and this is more of a general question about understanding antibiotic resistance of bacteria.
We are always told that careless antibiotics use is the cause of ...
43
votes
6
answers
12k
views
How do scientists kill the bacteria they themselves made resistant?
I was reading this article on researching bacteria resistance to silver by removing some of their genes.
Researchers then used "colony-scoring" software to measure the differences in growth and ...
- 499
0
votes
2
answers
157
views
Can you design a bacteriophage that attacks the part of the bacteria that makes them antibiotics resistant?
I read in a newspaper about a case where they had found a virus that could attack a specific form of antibiotics-resistant bacteria and managed to save a girl from a certain death by "infecting her" ...
- 590
0
votes
0
answers
42
views
Antibiotic resistant genes
Can someone help me find be it an article or journal that has the conventional PCR method for the detection of antibiotic resistant genes?
Thanks in advance.
- 27
3
votes
2
answers
365
views
If bacterial resistance randomly occur, then why limit broad-spectrum antibiotic use?
If there is importance to study some discipline, then one of the main matters is its applications, so besides the primary goal of knowing the truth of the matter regarding what that discipline is ...
- 399
2
votes
1
answer
92
views
Can (or have) antiviral drugs created drug-resistant viruses?
Evolution/emergence of antibiotic-resistance in bacteria is a known effect of extensive use of pharmaceutical antibiotics.
Pharmaceutical antivirals have come into extensive use in recent decades – e....
- 795
2
votes
1
answer
117
views
Loss of antibiotic resistance due to long term storage
How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
- 21
5
votes
1
answer
113
views
Are there limits to drug resistance?
It's well-known that indiscriminate use of a drug leads to resistance. My question is what the limits on resistance are. It seems obvious that there must be limits: for example I can hardly imagine a ...
- 537
2
votes
1
answer
21
views
How close are we to having phages take over the role of infection control from antibiotics? What developments need to occur?
The discovery of, and research in, the field of phages was overshadowed by the discovery and implementation of antibiotic treatments for infectious disease control. With the rising concern of ...
- 21
4
votes
1
answer
81
views
Why is AMR not widespread?
we know that resistant strains (MDR, XDR, PDR) have arisen in single incidents around the globe but why are these bacteria not common and not spreading quickly as humans keep using antibiotics and ...
- 154
1
vote
2
answers
85
views
Is it true that it is mostly impossible, for any kind of antibiotic, to succeed in killing off every bacteria in a very rich environment?
It is commonly believed that the resistance to antibiotics by micro-organisms is truly evolution at work, and that the recent surge in superbugs may very well be attributed to it.
When we refer to a ...
- 31
3
votes
2
answers
72
views
What effect does taking antibiotics unnecessarily have on future bacterial infections?
I have been told by my biology teacher that you shouldn't take antibiotics unnecessarily because "the body gets used to them" and it reduces the effectiveness of antibiotics against future bacterial ...
- 395
0
votes
1
answer
67
views
Is a wild-type strain more "fit" than its mutant strains?
Question's context
In the context of a presentation on mathematical models for antibiotic-resistance bacteria research, someone mentioned that wild type strain (WT) are expected to be more fit than ...
- 13
4
votes
1
answer
195
views
Test of antibiotic A for sensitivity as surrogate of antibiotic B
I am reading the WHO Global Antimicrobial Resistance Surveillance System (GLASS) - Manual for Early Implementation. it mentioned some of the drug-bug combination that will be put under surveillance in ...
- 113
-1
votes
2
answers
111
views
Why antimicrobial resistance is based mainly on antibiotic use? [closed]
Why antimicrobial resistance is based mainly on antibiotics use and not so much on antifungal or antivirus drugs? Why, when someone speaks about antimicrobial resistance, refers mostly to antibiotitic ...
- 1
3
votes
0
answers
120
views
Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?
Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
- 193
2
votes
3
answers
573
views
Could plasmids and conjugation mechanisms be used against antibiotic-resistant bacteria? [closed]
I'm surprised no one has mentioned something like this.
Plasmids are often exchanged between bacteria, sometimes through conjugation. In particular, conjugation could be considered an "open-port&...
- 765
1
vote
0
answers
25
views
How quickly do bacteria lose genes after selection has been removed? [duplicate]
If bacteria are subject to a biocide and then that biocide is removed from the population, how quickly will they lose that gene that encodes for resistance?
2
votes
1
answer
147
views
Why would eating the meat of cows fed with antibiotics trigger an immune response to the antibiotics? [closed]
The document "Antibiotics and Antibiotic resistance" contains the following paragraph:
The indiscriminate use of antibiotics in feed stuffs means that humans
may receive unwanted doses of ...
- 391
2
votes
1
answer
84
views
Have bacteria evolved antibiotic resistance in a laboratory setting?
Is it possible to breed a strain of bacteria in a laboratory setting so that it gains resistance to an antibiotic, replicating the kinds of resistance seen in wild, and has that been done?
- 123
0
votes
2
answers
52
views
What placement of plasmid parts makes the most sense?
If you want to express the largest amount of a certain or a few proteins using a plasmid, does it make sense for the antibiotic resistance gene to come after genes for proteins you intend to ...
- 371
2
votes
1
answer
767
views
Will dead cells in culture media have any significant impact on Live cells during incubation?
I am generating stable cell lines and have something like 25+ culture flasks to take care of. Ideally I can let dead cells sit in the media with the live adherent without rushing to change media ...
4
votes
2
answers
601
views
Resistance to Antiseptics
I know that bacteria can become resistant to antibiotics.
Why do antiseptics like alcohols, PVP-I, still work on the strains of bacteria we have today? Shouldn't some bacteria have evolved to be ...
- 41
-1
votes
1
answer
748
views
Drug and antibiotic resistance in organism [closed]
Out of gram positive or gram negative or both which one is having a higher resistance to drug or antibiotics? How the organism gains that gene for drug or antibiotics resistance.? Kindly explain it in ...
- 369
4
votes
1
answer
572
views
What does completing the course of antibiotics accomplish?
This article from the WHO states:
If you stop treatment early, there is a risk the antibiotics won’t have killed all the bacteria that made you sick and that it will mutate and become resistant. This ...
- 1,913
2
votes
0
answers
64
views
After a bacterial infection is dealt with, do any of that colony of bacteria remain?
Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
- 121
-2
votes
3
answers
2k
views
Why do we take antibiotics if our immune system already produces them? [closed]
Why do we take antibiotics if our immune system already produces them? Is it because our body doesnt make enough or the specific complementary antibody to fit with the antigen?
- 21
3
votes
1
answer
392
views
Why can bacteria resist topical antibiotics, but not compounds like Benzoyl Peroxide?
I excerpt from p 2 of 4, but p 4 of 4 reviews the entire article by Debra Jaliman, MD on Apr 17 2014.
Usually, topical antibiotics aren't recommended alone as an acne treatment, as they can increase ...
user4466
11
votes
1
answer
2k
views
Why don't vaccines cause bacterial resistance?
Since bacteria can evolve to overcome antibiotic use, why wouldn't they be able to evolve to overcome antibody or cell-mediated immunity?
(One possible explanation: antibiotics have only one target ...
- 971
3
votes
1
answer
658
views
Does long term use of antibiotics in humans actually lead to a greater risk of infection?
I've read about the overuse of antibiotics leading to antibiotic resistant strains of bacteria, so generally does long term use of antibiotics breed these strains in the bodies of antibiotic users and ...
- 876
1
vote
1
answer
576
views
Which immunosuppression caused by long-term usage of these antibiotics in untreated celiac disease?
These antibiotics include
amoxicillin + acidum clavulanicum (beta-lactam, inhibit cell wall synthesis, broad specturm, 3rd gen aminopenicillin) / last part preventing the resistance
ceftriaxone (3rd ...
- 5,149