Questions tagged [antibiotics]

Biologically-synthesized chemicals (or their derivatives) that cause growth suppression or death of bacteria. Other anti-microbials are often also considered under the class antibiotics these days.

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Does heterogeneous resistance have an impact on the zone diameter observed in disk diffusion susceptibility testing?

Question Does the presence of small, discrete colonies within the zone of inhibition, suggesting heterogeneous resistance, impact the zone diameter in disk diffusion susceptibility testing? "Help ...
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How is the zone of inhibition formed in disk diffusion?

Question How is the zone of inhibition formed in disk diffusion? What I mean by the formation of the zone of inhibition is not the ultimate visibility of such zone but the circumstances that result in ...
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Superbugs in eusocial animals

I am not a biologist, and I'm interested in eusocial animals, and the porous habitats in which they live. I know few facts about diseases in these animals: I know some interesting instances of viruses,...
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Does an antibiotic that inhibits protein synthesis delay bone generation?

After removal of a femoral intramedullary nail the bone needs to generate in the intramedullary canal and bone generation to take place at the screw sites. Would an antibiotic which inhibits protein ...
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Would it be possible to genetically modify a plant to produce usable antibodies? And if eaten would they be used?

I am thinking of a project where i would Gene edit a plants cells to produce anti bodies that humans could use. Does anyone know A. what genes to modify? B. Would the antibodies be usable after ...
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Is bacterial resistance caused by underdosing?

The phenomenon of antimicrobial resistance caused by overuse of antibiotics was predicted as early as 1945 by Alexander Fleming who said "The time may come when penicillin can be bought by anyone ...
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Can antibiotic resistance cause problems for the microorganisms producing them? [duplicate]

My understanding is that antibiotics are naturally produced by microorganisms as a defensive measure against certain kinds of bacteria. For example, some Penicillium molds produce penicillin. Clearly, ...
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How much Kanamycin is too much for selection in dh5α E. coli?

I'm using Kanamycin for selection after electroporation of my plasmid containing a kanamycin-resistance gene at a concentration of 50mg/L prepared in 1000x stock that is usually kept in frozen ...
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Does cell culturing contribute to dangerous antibiotic resistance to the same degree as livestock?

According to the CDC, antibiotic resistance is one of the biggest public health challenges of our time. The largest incubator for antibiotic resistance is the factory farming of animals where ...
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Nutrient agar as the medium for antibiotic susceptibility testing

I couldn't get Mueller Hinton agar to conduct antibiotic susceptibility testing. I'm thinking of just choosing nutrient agar as the growth medium for the bacteria Staphylococcus epidermidis. Will ...
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Antimicrobial susceptibility testing

To learn the Kirby-Bauer method, I've been looking at youtube videos where a bacteria's susceptibility is tested. In this video, the researcher places the discs (containing antibiotics) right after ...
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Which modern methods of antibiotic production are there?

For my seminar paper in the field of biology, I have to collect modern methods of antibiotic production. The topic is a little overwhelming, but the work should also be quite extensive (approx. 18 ...
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Can we develop a virus which is amicable for us but deadly for SARS-CoV-2 and HIV?

I am not biologist and do not have special education in it etc. It is know that in the wild, some species have their deadly enemies. What I am suggesting is can we humans find/create virus that is ...
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Can I find out what resistance bacteria has based on strain? where do I look for that info?

I have a list of strains across various organisms for example: Escherichia coli - ATCC 25922, CGSC 6152, W3110RL. Pseudomonas aeruginosa - ATCC 27853, PAO1, APAE1111. I'm failing to find any ...
Dominik Gurvic's user avatar
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If mutations are random, why do antibiotics 'cause' resistance?

Full disclosure: I am not a biologist and this is more of a general question about understanding antibiotic resistance of bacteria. We are always told that careless antibiotics use is the cause of ...
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Will all bacteria become resistant against all antibiotics in the long term?

(I asked the same at medicalsciences beta, but I expect a quite different perspective on it here) This is about bacteria that can infect humans, and their multi-resistance. The evolution of bacteria ...
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Selection marker for a cytoplasmic vector that works at low expression levels?

I need to express a selection marker on a plasmid in human cells (e.g. HEK293). However, I am using a cytoplasmic vector derived from a non-human virus with its own mechanism for transcriptional ...
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Why do bacteria adapt quickly to antibiotics but not to much more common threats?

People cook food all the time, why don't common bacteria adapt to extreme heat? It's winter in either hemisphere every year, why don't common bacteria adapt to extreme cold? People use soap or spray ...
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Why does tetracycline have such a broad spectrum of activity?

What are the structural and chemical characteristics that make tetracycline uniquely broad spectrum? I understand it acts on the A-site of the prokaryotic ribosome, but there exist many ribosome-...
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Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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Are red blood cells prokaryotic?

After searching "do antibiotics impact the immune system" I found out that antibiotics target prokaryotic cells. It all made a lot of sense thinking about all those yogurt recommendations you get ...
James Well's user avatar
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why don't antibiotics reduce our weight?

I know that a significant weight of the human body (in Kgs) is contributed by microbiota. I also know that antibiotics can often be broad spectrum and kill all the bacterias regardless of them being ...
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Why is AMR not widespread?

we know that resistant strains (MDR, XDR, PDR) have arisen in single incidents around the globe but why are these bacteria not common and not spreading quickly as humans keep using antibiotics and ...
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Technique to test antibiotic resistance in a bacteria in presence of another compound

so I have to 'quantitatively' assess a bacterial strain for its antibiotic resistance property (it is a specific antibiotic) in the presence and absence of another compound alongside antibiotic. What ...
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Is there any kind of antibiotic effective against fungi?

I know that antibiotics usually have properties affecting specifically bacterial cells, like by inhibiting peptidoglycan synthesis. but do any antibiotics exist affecting eukaryotic cells, like yeast ...
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What effect does taking antibiotics unnecessarily have on future bacterial infections?

I have been told by my biology teacher that you shouldn't take antibiotics unnecessarily because "the body gets used to them" and it reduces the effectiveness of antibiotics against future bacterial ...
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What does the "G" and "V" in penicillin V and penicillin G stand for?

I've been looking for a convincing explanation for the designation penicillin G and V. The answers I've found includes G as in "gold standard" and "V" as in "viscus" but they're not sourced and not ...
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Does cow urine have medicinal properties?

It is a common belief in India that cow urine has medicinal properties. It is said to be anti-biotic. It cures diabetes, cancer and many other diseases if not all. Because this is biology I would ...
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Infection treatment without anti-biotic

When we have some bacterial infection, say throat infection (pharyngitis) we take antibiotics as treatment. I was wondering how throat infection was treated when there was no antibiotics at all, ...
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Does Penicillium roqueforti produce penicyllin?

I have recently discovered that blue cheese is produced with Penicillium roqueforti. As I know that some Penicillinum sp. produce penicyllin I wonder whether it is produced by P. roqueforti too? The ...
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Microbroth dilution

I have performed MIC test for fungi candida albicans, Cryptococcus neoformans and Aspergillus fumigatus in a 96 well plate by broth microdilution method by drugs Amphotericin B and fluconazole.....
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Are fluoroquinolone antibiotics (Cipro, Levaquin, etc) chemotherapy drugs?

I read a claim (I can't find the source) that fluoroquinolone antibiotics are actually chemotherapy drugs, or maybe to be more specific, are "chemotherapeutic." Is this claim true? Additionally, if ...
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Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?

Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
user1803551's user avatar
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Could plasmids and conjugation mechanisms be used against antibiotic-resistant bacteria? [closed]

I'm surprised no one has mentioned something like this. Plasmids are often exchanged between bacteria, sometimes through conjugation. In particular, conjugation could be considered an "open-port&...
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How do the three mechanisms of the 1000X improved "new" vancomycin work together?

The BBC News article Ultra-tough antibiotic to fight superbugs says (in part): One hard-to-treat infection that has been worrying doctors is vancomycin-resistant enterococci or VRE. It has been found ...
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Why would eating the meat of cows fed with antibiotics trigger an immune response to the antibiotics? [closed]

The document "Antibiotics and Antibiotic resistance" contains the following paragraph: The indiscriminate use of antibiotics in feed stuffs means that humans may receive unwanted doses of ...
Mathematician's user avatar
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Why are aminoglycoside antibiotics particularly effective for aerobic gram negative bacteria?

Aminoglycosides are short chains of sugar molecules with some -OH groups substituted with amine groups. As far as I understand they function by causing bacterial ribosomes to misread RNA and create ...
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Why should benzalkonium chloride (an antifungal agent) be used during your laundry machine's "rinse" cycle, not its "wash" cycle?

I'll first provide lots of background information. My actual question is at the very end. Background The spread of athlete's foot Athlete's foot is a fungal infection which is mildly contagious. ...
tealhill supports Monica's user avatar
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Can methylisothiazolinone/methylchloroisothiazolinone (MI/MCI) behave as bacteriostatic in low concentrations?

Wikipedia mentions this as a biocide. It also mentions that low concentrations of certain bacteriocides is bacteriostatic. (I am assuming that biocide and bacteriocide play the same role, here.) ...
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Antibiotic resistance of Staphylococcus epidermidis and S. warneri

I am looking for information regarding the antibiotic resistance of S. epidermidis and S. warneri, specifically against penicillin, ampicillin and tetracycline but to no avail. Are there any good ...
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Have bacteria evolved antibiotic resistance in a laboratory setting?

Is it possible to breed a strain of bacteria in a laboratory setting so that it gains resistance to an antibiotic, replicating the kinds of resistance seen in wild, and has that been done?
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Is taking a shower unnecessary if good bacteria surround our skin?

David Whitlock, a scientist, has not taken a shower for 12 years. Instead, he sprays a bacterial mist containing live bacteria to remove odor and maintain the number of good bacteria. It is because ...
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Drug and antibiotic resistance in organism [closed]

Out of gram positive or gram negative or both which one is having a higher resistance to drug or antibiotics? How the organism gains that gene for drug or antibiotics resistance.? Kindly explain it in ...
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How do antibiotics combined with a non-Western diet affect weight loss?

Wikipedia describes a Western diet as: ... higher intakes of red and processed meat, butter, high-fat dairy products, eggs, refined grains, white potatoes, french fries, and high-sugar drinks. In ...
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What does completing the course of antibiotics accomplish?

This article from the WHO states: If you stop treatment early, there is a risk the antibiotics won’t have killed all the bacteria that made you sick and that it will mutate and become resistant. This ...
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Comparative effectiveness of antibiotics against colonies and growing bacteria

Appropriate antibiotics usually eliminate bacteria. However are the same antibiotics as effective against bacteria that have grown to form a colony?
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After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
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Why does azithromycin not affect human mitochondria?

Drugs like tetracyclines, macrolides and aminoglycosides bind to prokaryotic ribosomes. It is interesting that our body too having mitochondria, which have prokaryotic ribosomes, there is little(?) ...
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Why do we take antibiotics if our immune system already produces them? [closed]

Why do we take antibiotics if our immune system already produces them? Is it because our body doesnt make enough or the specific complementary antibody to fit with the antigen?
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Effective Mycoplasma Elimination from Primary Human Cultures

Obviously the best way to avoid mycoplasma contamination is to avoid it in the first place. In our case, however, it is not possible to avoid. We are culturing viruses and tissues out of human nasal ...
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